GB

Grădina Botanică Națională(Institut) "Alexandru Ciubotaru"

Laboratorul Embriologie şi Biotehnologie

                                                                                      Embryology and Biotechnology Laboratory

     Founded in 1964 by Acad. Alexander Ciubotaru and which served to foundation of embryology, cariology and cytogenetic science in our country. In Embryology Laboratory were carried out cariology, embryology, cytogenetic researches at the species of the sort: Zea, Triticum, Avena, Hordeum, Secale, Sorghum, Panicum, Tripsacum, Carex, Artemisia,Glycineetc.

      Concepts were developed:
  • Pollen grain-Pollen tube – the unique open bio-systems (A.Ciubotaru, 1965);
  • Homeostatic hypothesis of double fertilization (A. Ciubotaru, 1969,1970);
  • Neoplasmogenesis conception of zygote (A. Ciubotaru, 1972);
  • Morpho-functional status of gametogenesis (A. Ciubotaru, 1984);
  • Tapetogenesis concept (A. Ciubotaru, 1972 -1995);
  • Organogenesis (A. Ciubotaru, 1972 -1995);
  • Aromorfoza embryo (A. Ciubotaru 0.1972-2003).

    The first citoembriologic researches in Moldova were made in the mid-fifty (1954-1956) in the genetics section of Moldavian branch of Academy of sciences of the former URSS by a small group of enthusiasts S. Colesnicov , V. Krylov, A. Ciubotaru.

      In 1959 in the same section was created the cytology group (head of group A. Ciubotaru), which was transferred in 1964 to Botanical Garden of Academy of Sciences, and got started the foundation of cytology and embryology laboratory (1965). The years 1959 - 1963 were devoted to developing methods and properties of microtechnology and conducting karyological and embryological research to the level of photonic and electronic microscopy. Botanical Garden was among the first union institutions where embryological research was carried out at ultrastructural level. In the sixties were carried out karyological and embryological researches in a number of species of field crops, later (1970-1977), was investigated karyology of many spontaneous species widespread in Moldova. Investigations were focused on studying the structure and ultrastructure of reproductive cells. Monographs and collections on carried out researches were published.
   
    The current name given to laboratory was formed by merging of two divisions: Embryology, Anatomy of plants and plant biotechnology sector .
 
    Changing direction of research. Since 1996 the laboratory initiated a new direction of research in biotechnology, cytogenetics sphere on tissue culture and parasexual hybridization. Research is carried out on crops, ornamental and medicinal plants, most of which are traditional for Moldova, but in recent years, employees are concerned about the research of new plants species (allochthonous) for Moldova, which are of interest to the country's economy, whose regeneration is difficult on traditional way.
In time, new technologies were developed for micropropagation " in vitro " and "in vivo" and vigorous seedlings of Stevia rebaudiana Bertoni , Aerva lanata Juss., Polymnia sonchifolia Poepp. et endl., Rosmarinus officinalis L., Ceratostigma plumbaginoides Bunge, Withania somniferum L., Zizyphus jujube Mill., Ginkgo biloba L., Myrtus communis L., Actinidia deliciosa (Chev.) Liang, Ferguson were obtained.
    Laboratory staff have prepared and published 25 books, many scientific articles – about 700.0, 9 doctor habilitate theses and 25 doctoral theses were defended. In the laboratory, researchers in the field from other countries, such as: Azerbaijan, Georgia, Uzbekistan, Romania, Hungary, Bulgaria, Vietnam, took an internship.
 
    The scientific potential of laboratory and the work performed by employees are known and appreciated in the world. Scientific achievements were presented at multiple international and republican meetings. The laboratory was the organizer of the I International Symposium on "Electronic Microscopy and its Application in Botanical Studies," the V and IX Union Congresses on plant embryology.